Establishing Tissue Culture of King of Bitters (Andrographis paniculata) and Comparison of Callus Versus Plant Neoandrographolide Production

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dc.contributor.author Mihalko, Cassandra
dc.date.accessioned 2017-09-07T18:05:16Z
dc.date.available 2017-09-07T18:05:16Z
dc.date.issued 2017-09
dc.identifier.uri http://jewlscholar.mtsu.edu/xmlui/handle/mtsu/5351
dc.description.abstract Neoandrographolide is a secondary compound endemic to Andrographis paniculata (King of Bitters) and gives the plant its anti-inflammatory property. Because of the widespread use of neoandrographolide and the low seed germination rate of Andrographis paniculata, creating a callus tissue culture, from which to extract neoandrographolide, has the potential to be beneficial to pharmaceutical companies. Callus was produced from King of Bitters plant tissue using the Mineo protocol for tissue extraction and the Murashige protocol for the callus media and incubation. HPLC-CAD analysis was done on a liquid extract of callus (1 g dried callus to 50 mL methanol) and leaf tissue (1g dried leaves to 50 mL methanol) using the Li protocol. No detectable neoandrographolide content was found in the callus, but neoandrographolide was found in the plant leaf tissues. To facilitate neoandrographolide production in callus, elicitors, genetic manipulation, metabolic engineering, or the use of symbionts may be viable options. en_US
dc.publisher University Honors College, Middle Tennessee State University en_US
dc.subject King of Bitters en_US
dc.subject Andrographis paniculata en_US
dc.subject Andrographolide en_US
dc.subject neoandrographolide en_US
dc.subject tissue callus en_US
dc.subject HPLC-CAD en_US
dc.subject herbal medicine en_US
dc.title Establishing Tissue Culture of King of Bitters (Andrographis paniculata) and Comparison of Callus Versus Plant Neoandrographolide Production en_US
dc.type Thesis en_US


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