Adenosine Deaminating/ Hydrolyzing Enzymes from Alaska pea seeds (Pisum sativum)

Show simple item record

dc.contributor.advisor Kline, Paul
dc.contributor.author Thicklin, Lendsey Breanna
dc.date.accessioned 2017-10-04T20:17:48Z
dc.date.available 2017-10-04T20:17:48Z
dc.date.issued 2017-07-17
dc.identifier.uri http://jewlscholar.mtsu.edu/xmlui/handle/mtsu/5475
dc.description.abstract Nucleoside deaminases and nucleoside hydrolases are essential within the purine salvage pathway to recycle purine bases to promote cellular functions such as DNA synthesis, cytokinin metabolism, and more. Plants such as the Alaska pea (Pisum sativum) rely heavily on these enzymes, yet the enzymatic and structural characterizations of these proteins are limited. A 67 kilodalton (kDa) protein was isolated from germinated Alaska pea seeds with a 103 fold purification and 2.8 % recovery. The analysis of the enzymatic activity of this protein revealed adenosine is metabolized to inosine, adenine, and hypoxanthine suggesting a bifunctional enzyme combining nucleosidase and deaminase activities. This dimeric protein had an inosine Michaelis constant of 268 μM 78 μM. The optimum pH for enzymatic activity was determined to be pH 6. Research involving plant based nucleoside deaminases and nucleoside hydrolases can provide an origin of reference in the development of compounds that can be used as herbicides.
dc.publisher Middle Tennessee State University
dc.subject Biochemistry
dc.subject Deaminases
dc.subject Enzyme
dc.subject Nucleoside hydrolases
dc.subject Protein Purification
dc.title Adenosine Deaminating/ Hydrolyzing Enzymes from Alaska pea seeds (Pisum sativum)
dc.type Thesis
dc.contributor.committeemember Burden, Andrew
dc.contributor.committeemember Miller, Justin
dc.thesis.degreelevel Masters
dc.thesis.degreegrantor Middle Tennessee State University
dc.subject.umi Biochemistry
dc.subject.umi Chemistry
dc.description.degree M.S.
dc.contributor.department Chemistry


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search JEWLScholar@MTSU


Browse

My Account

Statistics