Strategy for Adapting Wine Yeasts for Bioethanol Production

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Date
2009-01-22
Authors
Ooi, Beng Guat
Lankford, Kevin R.
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Abstract
The Saccharomyces cerevisiae wine yeast strains 71B-1122 and K1-V1116 were used to derive strains that could tolerate and produce higher ethanol yields. Respiratory-deficient mutants resistant to 500 µg/mL lycorine were isolated. Two mutants, 71B-1122 YEBr L3 and K1-V1116 YEBr L4, were shown to achieve about 10% and 18% improvement in their glucose-to-ethanol conversion efficiency compared to their respective parent strains. The K1-V1116 YEBr L4 in particular can tolerate an ethanol yield of 18.8 ± 0.8% at 3.5 weeks of fermentation and continued to consume most of the sugar until less than 1% glucose was left.
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Keywords
Fermentation, Ethanol yield, Respiratory deficient mutants, Wine yeast, Llycorine resistance, Mitochondrial DNA
Citation
International Journal of Molecular Sciences. 2009 Jan 22;10(1):387
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