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AMINO ACID SEQUENCE OF NUCLEOSIDE HYDROLASES FROM PLANTS

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dc.contributor.advisor Kline, Paul en_US
dc.contributor.author Ogorodnik, Kateryna V. en_US
dc.date.accessioned 2014-08-28T18:35:38Z
dc.date.available 2014-08-28T18:35:38Z
dc.date.issued 2014-06-24 en_US
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/4274
dc.description.abstract Nucleoside hydrolases are key enzymes of the purine salvage pathways of various bacteria, yeast, parasitic protozoa, insects, fish, and plants. While the structures of nucleoside hydrolases from parasitic protozoans have been extensively studied, almost no structural information beyond subunit molecular weights is available for nucleoside hydrolases from plants. en_US
dc.description.abstract Based on a nonlinear regression using the Michaelis-Menten equation, the Michaelis constants (Km) of inosine for yellow lupin adenosine nucleosidase and inosine nucleosidase were determined. A Km of 260 75 μM for inosine nucleosidase and 9820 13801 μM for adenosine nucleosidase was found. The high standard deviation of the Km for adenosine nucleosidase was due to the solubility limits of inosine preventing a complete kinetic analysis. en_US
dc.description.abstract Three nucleoside hydrolases from plants, adenosine and inosine nucleosidase from yellow lupin, and adenosine nucleosidase from soybean, along with rihC from E. coli have been partially sequenced by mass spectrometry. After a final polishing step based on isoelectric focusing was performed, the enzymes were subjected to tryptic digestion followed by separation of the resulting peptides using reverse phase HPLC. The peptides were subjected to MS/MS and the sequence of selected peptides determined using PEAKS software. en_US
dc.publisher Middle Tennessee State University en_US
dc.title AMINO ACID SEQUENCE OF NUCLEOSIDE HYDROLASES FROM PLANTS en_US
dc.type Thesis en_US
dc.contributor.committeemember Burden, Donald en_US
dc.contributor.committeemember Farone, Mary en_US
dc.thesis.degreelevel Masters en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.subject.umi Chemistry en_US
dc.subject.umi Biochemistry en_US
dc.description.degree M.S. en_US
dc.contributor.department Chemistry en_US


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