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Investigating the Use of Ralstonia eutropha's H2-Sensing Pathway in a Heterologous Biological H2-Sensing Reporter

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dc.contributor.advisor Robertson, Brian en_US
dc.contributor.author Havlik, Lawrence Patrick en_US
dc.date.accessioned 2015-06-12T19:07:42Z
dc.date.available 2015-06-12T19:07:42Z
dc.date.issued 2015-04-09 en_US
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/4484
dc.description.abstract Increasing interest in alternative fuels has driven increasing interest in en_US
dc.description.abstract biologically derived hydrogen, such as that produced by phototrophic microorganisms like cyanobacteria and green microalgae. The goal of this study was to test whether the hydrogen-sensing pathway of the soil bacteria Ralstonia eutropha could drive expression of a luciferase in a hydrogen-dependent manner so as to form a heterologous biological hydrogen-sensing reporter. Plasmids containing the hox and hyp clusters of genes reported to be necessary for hydrogen sensing in R. eutropha were introduced into Escherichia coli. However, the lack of bioluminescence in response to hydrogen demonstrated that those genes were not sufficient to serve as a hydrogen-sensitive transcription regulator in E. coli. This indicates that there is some factor not currently described that is necessary for the system's proper functioning in R. eutropha. The idea of a heterologous hydrogen-sensing reporter could be revisited once the pathways have become more fully characterized. en_US
dc.publisher Middle Tennessee State University en_US
dc.subject Biohydrogen en_US
dc.subject Hydrogenase en_US
dc.subject Ralstonia eutropha en_US
dc.title Investigating the Use of Ralstonia eutropha's H2-Sensing Pathway in a Heterologous Biological H2-Sensing Reporter en_US
dc.type Thesis en_US
dc.contributor.committeemember Elrod-Erickson, Matthew en_US
dc.contributor.committeemember Altman, Elliot en_US
dc.thesis.degreelevel Masters en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.subject.umi Biology en_US
dc.subject.umi Genetics en_US
dc.subject.umi Molecular biology en_US
dc.description.degree M.S. en_US
dc.contributor.department Biology en_US


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