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DEGRADATION OF PENTOBARBITAL IN VARIOUS SOIL TYPES BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY / MASS SPECTROMETRY

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dc.contributor.advisor Kline, Paul
dc.contributor.author Saha, Anita
dc.date.accessioned 2016-08-15T15:07:38Z
dc.date.available 2016-08-15T15:07:38Z
dc.date.issued 2016-07-12
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/5038
dc.description.abstract Pentobarbital is a leading drug for euthanizing large farm animals [Wolfgang et al., 2009]. However, pentobarbital tends to leach into the surrounding soil and become a source of contamination once these euthanized animals are buried. This research was conducted to determine the breakdown rate and extraction efficiency of pentobarbital adsorbed in different types of soil. Additional studies include examining a microbe strain possessing an enzyme capable of breaking down pentobarbital into its metabolites that has leached into the soil.
dc.description.abstract Solid phase extraction coupled with LC/MS was an efficient method for detecting and quantifying pentobarbital from complex matrices, such as soil. The established method was capable of quantifying 0.5 g of pentobarbital per gram of soil (500 ppb). The soils were spiked with desired amount of pentobarbital and were analyzed daily and weekly to understand the degradation pattern of pentobarbital. In addition, soil samples were autoclaved at 121C to determine if any bacteria caused the degradation of pentobarbital in the soil samples. The finding suggests that the degradation of pentobarbital can be due to microbial influences or nature of the soil or possibly both.
dc.publisher Middle Tennessee State University
dc.title DEGRADATION OF PENTOBARBITAL IN VARIOUS SOIL TYPES BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY / MASS SPECTROMETRY
dc.type Thesis
dc.contributor.committeemember Burden, Donald
dc.contributor.committeemember Farone, Mary
dc.thesis.degreelevel Masters
dc.thesis.degreegrantor Middle Tennessee State University
dc.subject.umi Chemistry
dc.description.degree M.S.
dc.contributor.department Chemistry en_US


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