Isolation and Identification of Possible Atrazine Degraders from Middle Tennessee Soils

dc.contributor.advisor DuBois, John en_US Griffith, Misty en_US
dc.contributor.committeemember Farone, Mary en_US
dc.contributor.committeemember Seipelt-Thiemann, Rebecca en_US
dc.contributor.department Biology en_US 2014-12-19T19:05:52Z 2014-12-19T19:05:52Z 2014-10-30 en_US
dc.description.abstract Organisms that were potential degraders of atrazine were tested for atrazine tolerance by inoculation onto tryptic soy atrazine agar (TSAA). The atrazine-tolerant organisms were plated onto minimal salts atrazine agar, and compared to growth on minimal salts agar. Six soil samples were taken from various farm land sites in middle Tennessee. Soils had known repeated atrazine use, but had not been sampled for atrazine degraders. Soil isolates were identified using the BBL CrystalTN Identification System. Carbon dioxide (CO2) evolution studies and atrazine enrichment studies were conducted. Six isolates and four known bacteria showed promising 5 day averages of CO2 production, all above 20 of CO2 while in the presence of atrazine. Enrichment procedures showed growth, for 5 out of 6 soil isolates with no glucose and 2.65 mL per 250 mL of medium, indicating their reliance on atrazine for growth. Soil samples taken from middle Tennessee showed organisms with atrazine tolerance and potential atrazine degradation. en_US M.S. en_US
dc.publisher Middle Tennessee State University en_US
dc.subject.umi Biology en_US
dc.subject.umi Macroecology en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.thesis.degreelevel Masters en_US
dc.title Isolation and Identification of Possible Atrazine Degraders from Middle Tennessee Soils en_US
dc.type Thesis en_US
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