Demonstration of the utility of a dual-color luciferase reporter in S. cerevisiae

dc.contributor.advisor Robertson, James en_US Krishnamoorthy, Archana en_US
dc.contributor.committeemember Erickson, Matthew en_US
dc.contributor.committeemember Jessen, Jason en_US
dc.contributor.department Biology en_US 2015-06-12T19:07:42Z 2015-06-12T19:07:42Z 2015-06-01 en_US
dc.description.abstract A reporter gene helps determine the regulation of a gene of interest by producing a protein product that can be easily detected when the gene of interest is active. The luciferase gene from click beetles can be used as a reporter because its protein product emits light that can be measured. Monitoring transcription by luciferase activity is hampered in fast growing organisms due to sudden changes in oxygen concentration and cell number as these variables also affect light emission. This study uses green luciferase to report transcription of a gene of interest and red luciferase to simultaneously report activity of a reference gene. The ratio of these colors can (i) mitigate sudden changes in rapid growth conditions, and (ii) be used to predict productivity of the cell population during processes such as the Yeast Respiratory Oscillation. We envision this tool to provide valuable insights into genetic control in S. cerevisiae. en_US M.S. en_US
dc.publisher Middle Tennessee State University en_US
dc.subject Luciferase en_US
dc.subject Reporter gene en_US
dc.subject S. cerevisiae en_US
dc.subject Yeast Respiratory Oscillation en_US
dc.subject.umi Cellular biology en_US
dc.subject.umi Molecular biology en_US
dc.subject.umi Biology en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.thesis.degreelevel Masters en_US
dc.title Demonstration of the utility of a dual-color luciferase reporter in S. cerevisiae en_US
dc.type Thesis en_US
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