Demonstration of the utility of a dual-color luciferase reporter in S. cerevisiae

dc.contributor.advisorRobertson, Jamesen_US
dc.contributor.authorKrishnamoorthy, Archanaen_US
dc.contributor.committeememberErickson, Matthewen_US
dc.contributor.committeememberJessen, Jasonen_US
dc.contributor.departmentBiologyen_US
dc.date.accessioned2015-06-12T19:07:42Z
dc.date.available2015-06-12T19:07:42Z
dc.date.issued2015-06-01en_US
dc.description.abstractA reporter gene helps determine the regulation of a gene of interest by producing a protein product that can be easily detected when the gene of interest is active. The luciferase gene from click beetles can be used as a reporter because its protein product emits light that can be measured. Monitoring transcription by luciferase activity is hampered in fast growing organisms due to sudden changes in oxygen concentration and cell number as these variables also affect light emission. This study uses green luciferase to report transcription of a gene of interest and red luciferase to simultaneously report activity of a reference gene. The ratio of these colors can (i) mitigate sudden changes in rapid growth conditions, and (ii) be used to predict productivity of the cell population during processes such as the Yeast Respiratory Oscillation. We envision this tool to provide valuable insights into genetic control in S. cerevisiae.en_US
dc.description.degreeM.S.en_US
dc.identifier.urihttp://jewlscholar.mtsu.edu/handle/mtsu/4486
dc.publisherMiddle Tennessee State Universityen_US
dc.subjectLuciferaseen_US
dc.subjectReporter geneen_US
dc.subjectS. cerevisiaeen_US
dc.subjectYeast Respiratory Oscillationen_US
dc.subject.umiCellular biologyen_US
dc.subject.umiMolecular biologyen_US
dc.subject.umiBiologyen_US
dc.thesis.degreegrantorMiddle Tennessee State Universityen_US
dc.thesis.degreelevelMastersen_US
dc.titleDemonstration of the utility of a dual-color luciferase reporter in S. cerevisiaeen_US
dc.typeThesisen_US

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