Characterization of Open Reading Frames in the Tobacco Mitochondrial Transcriptome

dc.contributor.advisor Cahoon, Aubrey en_US Grimes, Ben en_US
dc.contributor.committeemember Elrod-Erickson, Matthew en_US
dc.contributor.committeemember Seipelt-Thiemann, Rebecca en_US
dc.contributor.department Biology en_US 2014-06-02T18:50:37Z 2014-06-02T18:50:37Z 2013-06-28 en_US
dc.description.abstract Initial annotation of the tobacco mitochondrial genome in 2005 identified numerous identifiable protein coding, tRNA, and rRNA genes plus 119 open reading frames (ORFs) with no clearly stated function. The purpose of this study was to explore expression of putative open reading frames in the Nicotiana tabacum mitochondrial genome. I surmised that if these gene products are transcribed and translated they may have a functional role in the tobacco mitochondria. In this study, these ORFs were screened for expression using RNA deep sequencing, qRT-PCR, and polysome analysis. Twenty-five of the 119 were found to have steady-state RNA amounts above background and 15 of those were found to be polysome associated. These ORFs were also bioinformatically compared to other genes. Some of the transcribed ORFs were found to be fragments of mitochondrial proteins, some were not identifiable while some were homologous to plastid or nuclear coding genes. Five of the expressed ORFs have been linked to cytoplasmic male sterility in other plants. The 10 remaining ORFs found to be polysome-associated have the potential to be novel mitochondrial proteins. en_US M.S. en_US
dc.publisher Middle Tennessee State University en_US
dc.subject Expression en_US
dc.subject Genome en_US
dc.subject Mitochondria en_US
dc.subject Open Reading Frames en_US
dc.subject Transcriptome en_US
dc.subject.umi Plant biology en_US
dc.subject.umi Molecular biology en_US
dc.subject.umi Genetics en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.thesis.degreelevel Masters en_US
dc.title Characterization of Open Reading Frames in the Tobacco Mitochondrial Transcriptome en_US
dc.type Thesis en_US
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