DETERMINATION OF THE CONFORMATION OF SPECIFIC RESIDUES IN A MODEL PEPTIDE BY <super>13</super>C ISOTOPE-EDITED ATR-FTIR IN REGULAR WATER

dc.contributor.advisorWang, Chengshanen_US
dc.contributor.authorPOTANA, SNEHAen_US
dc.contributor.committeememberChong, Ngeeen_US
dc.contributor.committeememberDing, Keyingen_US
dc.contributor.departmentChemistryen_US
dc.date.accessioned2014-06-02T18:55:07Z
dc.date.available2014-06-02T18:55:07Z
dc.date.issued2014-01-01en_US
dc.description.abstractDetermination of protein structures has attracted extensive scientific attention. IR spectroscopy using traditional liquid cells has been reported to address the structure in a model peptide of sequence Ac-YAAKAAAKAAAAKAAH-NH<sub>2</sub> (pep17) with <super>13</super>C isotopic labels in deuterated water (D<sub>2</sub>O). Although similar to regular water, D<sub>2</sub>O is not physiologically equivalent to H<sub>2</sub>O in the aqueous matrix of cells and tissues. Therefore, peptide studies in D<sub>2</sub>O may not yield true peptide conformations. Here, <super>13</super>C isotope-edited FTIR was applied on pep17 in H<sub>2</sub>O using the Attenuated Total Reflection (ATR) technique. The peak of <super>13</super> C labeled residues in &#945;-helix appears at 1602 cm<super>-1</super>, which can be used as a fingerprint peak of &#945;-helix for the <super>13</super>C labeled residue. By the presence or absence of peak at 1602 cm<super>-1</super>, conformation of specific residues in the peptide was determined. For the first time, the elucidation of the secondary structure of the pep17 has been demonstrated in H<sub>2</sub>O.en_US
dc.description.degreeM.S.en_US
dc.identifier.urihttp://jewlscholar.mtsu.edu/handle/mtsu/3587
dc.publisherMiddle Tennessee State Universityen_US
dc.subjectAmino acidsen_US
dc.subjectCDen_US
dc.subjectFTIRen_US
dc.subjectIsotopeen_US
dc.subjectPeptideen_US
dc.subject.umiChemistryen_US
dc.thesis.degreegrantorMiddle Tennessee State Universityen_US
dc.thesis.degreelevelMastersen_US
dc.titleDETERMINATION OF THE CONFORMATION OF SPECIFIC RESIDUES IN A MODEL PEPTIDE BY <super>13</super>C ISOTOPE-EDITED ATR-FTIR IN REGULAR WATERen_US
dc.typeThesisen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
POTANA_mtsu_0170N_10166.pdf
Size:
1.41 MB
Format:
Adobe Portable Document Format

Collections