Identification of Tumor Necrosis Factor-α Inhibitors from Traditionally Used Medicinal Plants

dc.contributor.advisor Farone, Anthony Garrison, Meagan Elizabeth
dc.contributor.committeemember Farone, Mary
dc.contributor.committeemember Wright, Stephen
dc.contributor.department Biology en_US 2017-05-26T17:45:50Z 2017-05-26T17:45:50Z 2017-05-08
dc.description.abstract The inflammatory response protects our bodies from foreign pathogens. However, when inflammation is uncontrolled it can lead to inflammatory and autoimmune diseases. The drugs currently used as treatment for these diseases, such as non-steroidal anti-inflammatory drugs (NSAIDs), have very serious side effects. There is a need for less toxic, alternative treatments. Traditional Chinese Medicine is becoming more popular in the United States and many plants are being evaluated for their medicinal purposes. In this study, 12 subfractions of one primary extract from a particular Chinese plant, designated 25A, and 44 fractions of a local plant, Cichorium intybus (chicory), were screened for cytotoxicity to human macrophage-like cells and the non-toxic concentrations were then assayed for TNF-α inhibitory activity. Fraction 7 of 25A exhibited greater than 90% inhibition of TNF-α while simultaneously having lower than 10% toxicity to THP-1 human macrophage-like cells and was further separated into three pure fractions. 25A-7 F1 was the only fraction to show greater than 90% TNF-α inhibition. The same process was repeated with the 44 chicory samples from four sources: wild-type, hairy root, and callus cell culture with and without elicitors. The callus cell culture subfractions showed no inhibitory activity. The remaining two sources, wild-type and hairy root, had promising activity with various fractions producing TNF-α inhibition. This research provides potential anti-inflammatory treatment candidates from medicinal plants. M.S.
dc.publisher Middle Tennessee State University
dc.subject Drug discovery
dc.subject Inflammation
dc.subject Inflammatory disease
dc.subject Macrophages
dc.subject Traditional Chinese Medicine
dc.subject Tumor Necrosis Factor
dc.subject.umi Biology
dc.subject.umi Immunology
dc.thesis.degreegrantor Middle Tennessee State University
dc.thesis.degreelevel Masters
dc.title Identification of Tumor Necrosis Factor-α Inhibitors from Traditionally Used Medicinal Plants
dc.type Thesis
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