High Throughput Screening of Extracts From Plants Used in Traditional Chinese Medicine Against Trypanosoma brucei brucei 427

dc.contributor.advisor Newsome, Anthony en_US
dc.contributor.author Floyd, Michael Ryan en_US
dc.contributor.committeemember Ervin, Gore en_US
dc.contributor.committeemember Dubois, John en_US
dc.contributor.department Biology en_US
dc.date.accessioned 2014-06-02T18:44:39Z
dc.date.available 2014-06-02T18:44:39Z
dc.date.issued 2013-03-26 en_US
dc.description.abstract Trypanosoma brucei, the protozoan blood parasite, is the etiological agent for African trypanosomiasis (also known as "sleeping sickness"). This parasite is estimated to infect almost 30,000 people each year in central Africa, and has a 100% mortality rate if left untreated. The current drugs used to treat infection by T. brucei were developed almost a century ago. These compounds are toxic, expensive, and are becoming ineffective due to increased resistance. There are significant needs for new drug therapies for the treatment of T. brucei infections. The purpose of this study was to screen plants used in traditional Chinese medicine for activity against T. brucei. A library of 144 crude extracts from 34 different plants was screened against the trypanosomes using high throughput screening techniques and a resazurin based PrestoBlue assay. The extract cytotoxicity was also evaluated using L6 rat skeletal myoblast cells. The chloroform and water extracts of Scutellaria baicalensis showed the highest activity against the trypanosomes (IC50 of 11.43 and 19.86 μg/ml) as well as selectivity for the trypanosomes over the mammalian cells. The petroleum ether extracts of Psychotria rubra, and Elephantopus scaber, the ethyl acetate extract of Pandanus tectorius, and the extract of Belamcanda chinensis prepared with 95% ethanol all showed promising activity (IC50 of 38.02 - 49.6 μg/ml) and high selectivity. The results suggest that plants used in traditional Chinese medicine may have biochemical compounds of potential interest in the search for better drugs to treat African sleeping sickness. en_US
dc.description.degree M.S. en_US
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/3498
dc.publisher Middle Tennessee State University en_US
dc.subject Ethnomedicine en_US
dc.subject Trypanosoma brucei en_US
dc.subject.umi Parasitology en_US
dc.subject.umi Microbiology en_US
dc.subject.umi Biology en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.thesis.degreelevel Masters en_US
dc.title High Throughput Screening of Extracts From Plants Used in Traditional Chinese Medicine Against Trypanosoma brucei brucei 427 en_US
dc.type Thesis en_US
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