Spermatogonial stem cell transfer to a mule

dc.contributor.advisor Haffner, John
dc.contributor.author Smith, Emily Ann
dc.contributor.committeemember Hoffman, Rhonda
dc.contributor.committeemember Spooner, Holly
dc.contributor.department Agribusiness & Agriscience en_US
dc.date.accessioned 2015-12-18T19:10:32Z
dc.date.available 2015-12-18T19:10:32Z
dc.date.issued 2015-11-01
dc.description.abstract Spermatogonial stem cell transfer has been studied in various species and suggested potential for success. Bull, stallion, donkey, mule, boar, dog and cat testicles were examined using various injection techniques to determine distribution patterns of injected material most favorable for stem cell transfer. Spermatogonial stem cells were recovered from freshly harvested stallion testicles using enzymatic digestion and filtration and placed in suspension for transplantation. An 18 mo old sexually intact male mule was anesthetized and the stem cell suspension was injected into both testicles in the area below the head of the epididymis. Semen collected from the mule four months post-transplantation contained no spermatozoa. The stem cell transfer was unsuccessful in this case, however further research is needed to determine recipient age of injection to optimize donor cell development.
dc.description.degree M.S.
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/4749
dc.publisher Middle Tennessee State University
dc.subject Germ cell transfer
dc.subject Mules
dc.subject Spermatogenesis
dc.subject Spermatogonial stem cell trans
dc.subject.umi Animal sciences
dc.thesis.degreegrantor Middle Tennessee State University
dc.thesis.degreelevel Masters
dc.title Spermatogonial stem cell transfer to a mule
dc.type Thesis
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