Evaluation of extracts used in traditional Chinese medicine for antiviral potential against herpes simplex virus type 1

dc.contributor.advisor Wright, Stephen en_US
dc.contributor.author House, Megan Leigh en_US
dc.contributor.committeemember Farone, Mary en_US
dc.contributor.committeemember Newsome, Anthony en_US
dc.contributor.department Biology en_US
dc.date.accessioned 2014-06-02T18:55:06Z
dc.date.available 2014-06-02T18:55:06Z
dc.date.issued 2013-10-24 en_US
dc.description.abstract Herpes Simplex Virus type 1 (HSV-1) is a common pathogen that causes disease throughout the world. The need for new methods for the control and prevention of the virus is vital to reduce the number of people affected by HSV-1. A recent review concluded that natural products represent an important source for new antiviral activity and that Traditional Chinese Medicines (TCMs) are good sources for these agents. The objective of this study was to determine if any TCM plant extracts have potential active compounds capable of antiviral activity against HSV-1, with the ultimate goal identifying anti-HSV-1 drug candidates. After performing a cytotoxicity screen for each of 140 TCM extracts, Vero cells were exposed to HSV-1 and one of the extracts simultaneously to determine antiviral potential. Antiviral potential was determined by fluorescence readings from a spectrophotometer taken after a period of virus, cell, and extract incubation. Cell viability was determined using the fluorescent dye PrestoBlue which is reduced to a fluorescent red color by viable cells. Ten extracts showed potential antiviral activity by maintaining cell viability though cells were infected with HSV-1. The most effective four extracts inhibited HSV-1 by 80% and included Mussaenda pubescens, Antirrhinum majus, Bidens biternata, and Gnetum parvifolium. Further testing will be done to isolate active compounds from these extracts. en_US
dc.description.degree M.S. en_US
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/3581
dc.publisher Middle Tennessee State University en_US
dc.subject Herpes en_US
dc.subject HSV-1 en_US
dc.subject Plant extract en_US
dc.subject Traditional Chinese Medicine en_US
dc.subject Virus en_US
dc.subject.umi Biology en_US
dc.subject.umi Virology en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.thesis.degreelevel Masters en_US
dc.title Evaluation of extracts used in traditional Chinese medicine for antiviral potential against herpes simplex virus type 1 en_US
dc.type Thesis en_US
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