Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions

dc.contributorMiddle Tennessee State University. Biology Department.en_US
dc.contributor.authorGrimes, Benjamin Ten_US
dc.contributor.authorSisay, Awa Ken_US
dc.contributor.authorCarroll, Hyrum Den_US
dc.contributor.authorCahoon, A Bruceen_US
dc.date.accessioned2014-06-24T15:32:02Z
dc.date.available2014-06-24T15:32:02Z
dc.date.issued2014-01-13en_US
dc.description.abstractBackground: The purpose of this study was to sequence and assemble the tobacco mitochondrial transcriptome and obtain a genomic-level view of steady-state RNA abundance. Plant mitochondrial genomes have a small number of protein coding genes with large and variably sized intergenic spaces. In the tobacco mitogenome these intergenic spaces contain numerous open reading frames (ORFs) with no clear function.en_US
dc.description.abstractResults: The assembled transcriptome revealed distinct monocistronic and polycistronic transcripts along with large intergenic spaces with little to no detectable RNA. Eighteen of the 117 ORFs were found to have steady-state RNA amounts above background in both deep-sequencing and qRT-PCR experiments and ten of those were found to be polysome associated. In addition, the assembled transcriptome enabled a full mitogenome screen of RNA C→U editing sites. Six hundred and thirty five potential edits were found with 557 occurring within protein-coding genes, five in tRNA genes, and 73 in non-coding regions. These sites were found in every protein-coding transcript in the tobacco mitogenome.
dc.description.abstractConclusion: These results suggest that a small number of the ORFs within the tobacco mitogenome may produce functional proteins and that RNA editing occurs in coding and non-coding regions of mitochondrial transcripts.
dc.identifier.citationBMC Genomics. 2014 Jan 13;15:31en_US
dc.identifier.urihttp://jewlscholar.mtsu.edu/handle/mtsu/4232
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectMitochondrial transcriptomeen_US
dc.subjectPlant mitogenomeen_US
dc.subjectTobaccoen_US
dc.subjectNicotiana tabacumen_US
dc.subjectRNA editingen_US
dc.titleDeep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regionsen_US
dc.typeResearch Articleen_US

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