Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions
dc.contributor | Middle Tennessee State University. Biology Department. | en_US |
dc.contributor.author | Grimes, Benjamin T | en_US |
dc.contributor.author | Sisay, Awa K | en_US |
dc.contributor.author | Carroll, Hyrum D | en_US |
dc.contributor.author | Cahoon, A Bruce | en_US |
dc.date.accessioned | 2014-06-24T15:32:02Z | |
dc.date.available | 2014-06-24T15:32:02Z | |
dc.date.issued | 2014-01-13 | en_US |
dc.description.abstract | Background: The purpose of this study was to sequence and assemble the tobacco mitochondrial transcriptome and obtain a genomic-level view of steady-state RNA abundance. Plant mitochondrial genomes have a small number of protein coding genes with large and variably sized intergenic spaces. In the tobacco mitogenome these intergenic spaces contain numerous open reading frames (ORFs) with no clear function. | en_US |
dc.description.abstract | Results: The assembled transcriptome revealed distinct monocistronic and polycistronic transcripts along with large intergenic spaces with little to no detectable RNA. Eighteen of the 117 ORFs were found to have steady-state RNA amounts above background in both deep-sequencing and qRT-PCR experiments and ten of those were found to be polysome associated. In addition, the assembled transcriptome enabled a full mitogenome screen of RNA C→U editing sites. Six hundred and thirty five potential edits were found with 557 occurring within protein-coding genes, five in tRNA genes, and 73 in non-coding regions. These sites were found in every protein-coding transcript in the tobacco mitogenome. | |
dc.description.abstract | Conclusion: These results suggest that a small number of the ORFs within the tobacco mitogenome may produce functional proteins and that RNA editing occurs in coding and non-coding regions of mitochondrial transcripts. | |
dc.identifier.citation | BMC Genomics. 2014 Jan 13;15:31 | en_US |
dc.identifier.uri | http://jewlscholar.mtsu.edu/handle/mtsu/4232 | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_US |
dc.subject | Mitochondrial transcriptome | en_US |
dc.subject | Plant mitogenome | en_US |
dc.subject | Tobacco | en_US |
dc.subject | Nicotiana tabacum | en_US |
dc.subject | RNA editing | en_US |
dc.title | Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions | en_US |
dc.type | Research Article | en_US |
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