Overcoming the Recalcitrance for the Conversion of Kenaf Pulp to Glucose via Microwave-Assisted Pre-Treatment Processes

dc.contributor Middle Tennessee State University. Department of Chemistry en_US
dc.contributor.author Ooi, Beng Guat en_US
dc.contributor.author Rambo, Ashley L. en_US
dc.contributor.author Hurtado, Miguel A. en_US
dc.date.accessioned 2014-06-24T15:34:42Z
dc.date.available 2014-06-24T15:34:42Z
dc.date.issued 2011-02-21 en_US
dc.description.abstract This study evaluates the pre-treatment of cellulose from kenaf plant to yield sugar precursors for the production of ethanol or butanol for use as biofuel additives. In order to convert the crystalline cellulosic form to the amorphous form that can undergo enzymatic hydrolysis of the glycosidic bond to yield sugars, kenaf pulp samples were subjected to two different pre-treatment processes. In the acid pre-treatment, the pulp samples were treated with 37.5% hydrochloric acid in the presence of FeCl3 at 50 °C or 90 °C whereas in the alkaline method, the pulp samples were treated with 25% sodium hydroxide at room temperature and with 2% or 5% sodium hydroxide at 50 °C. Microwave-assisted NaOH-treatment of the cellulose was also investigated and demonstrated to be capable of producing high glucose yield without adverse environmental impact by circumventing the use of large amounts of concentrated acids i.e., 83-85% phosphoric acid employed in most digestion processes. The treated samples were digested with the cellulase enzyme from Trichoderma reesei. The amount of glucose produced was quantified using the Quantichrom glucose bioassay for assessing the efficiency of glucose production for each of the treatment processes. The microwave-assisted alkaline pre-treatment processes conducted at 50 °C were found to be the most effective in the conversion of the crystalline cellulose to the amorphous form based on the significantly higher yields of sugar produced by enzymatic hydrolysis compared to the untreated sample. en_US
dc.identifier.citation International Journal of Molecular Sciences. 2011 Feb 21;12(3):1451 en_US
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/4236
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.subject Cellulose en_US
dc.subject Crystalline en_US
dc.subject Lignin en_US
dc.subject Cellulase en_US
dc.subject Ethanol en_US
dc.subject Pre-treatment en_US
dc.subject Glucose en_US
dc.subject Kenaf fiber en_US
dc.subject Avicel en_US
dc.subject Microwave en_US
dc.title Overcoming the Recalcitrance for the Conversion of Kenaf Pulp to Glucose via Microwave-Assisted Pre-Treatment Processes en_US
dc.type Research Article en_US
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