An Evolutionary Analysis of Membrane-Associated Guanylate Kinase Protein Family

dc.contributor.authorCarr, Ryan
dc.contributor.authorDeal, Heather
dc.contributor.authorSeipelt-Thiemann, Rebecca
dc.contributor.departmentBiologyen_US
dc.date.accessioned2016-08-04T12:50:16Z
dc.date.available2016-08-04T12:50:16Z
dc.date.issued2016
dc.description.abstractGene families come into being through gene and/or genome duplication followed by mutation over time which results in the evolutionarily-related genes having somewhat different nucleotides, amino acids, gene structure, and functions. The membrane-associated guanylate kinase protein family has twelve members in humans: DLG1, DLG2, DLG3, DLG4, CASK, MPP1, MPP2, MPP3, MPP4, MPP5, MPP6, and MPP7. This gene/protein family is characterized by the presence of three specific protein domains: PDZ, SH3, and GUK, all of which aid in protein-protein interactions. These proteins are known to interact with cytoskeletal proteins and also are involved in signal transduction. A characteristic member of this family is the DLG3 gene, is responsible for encoding a synapse associated protein (SAP102). The goal of this study was to better understand the evolutionary relationships among the protein/gene family members. To attain this goal, two evolutionary investigations were undertaken. First, phylogenetic trees, which are the traditional method of analysis, were constructed using the amino acids. This analysis indicated evidence for three distinct sub-groups: group A contained CASK, MPP1, MPP2, MPP6; group B contained MPP3, MPP4, MPP5, MPP7; and group C contained DLG 1, DLG2, DLG3, DLG4. Next, the phylogenetic relationship based on the exon structure was undertaken. Briefly, multiple alignments were combined exon boundary information to generate a visual map of similarities and differences in exon structure among the gene family members. This visualization and its comparison to the traditional phylogenetic analysis will be presented.en_US
dc.identifier.urihttp://jewlscholar.mtsu.edu/handle/mtsu/4958
dc.language.isoen_USen_US
dc.publisherMiddle Tennessee State Universityen_US
dc.subjectDLGen_US
dc.subjectDisks Large Homologen_US
dc.subjectguanylateen_US
dc.subjectkinaseen_US
dc.subjectgeneen_US
dc.subjectgene familyen_US
dc.subjectprotein familyen_US
dc.subjectMolecularen_US
dc.subjectEvolutionaryen_US
dc.subjectGeneticsen_US
dc.subjectAnalysisen_US
dc.subjectMega 6en_US
dc.titleAn Evolutionary Analysis of Membrane-Associated Guanylate Kinase Protein Familyen_US
dc.typePresentationen_US

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