EXTRACTION AND DETECTION OF PENTOBARBITAL IN SOIL BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY / MASS SPECTROMETRY

dc.contributor.advisor Kline, Paul en_US
dc.contributor.author Bagsby, Chasity Diane en_US
dc.contributor.committeemember Farone, Anthony en_US
dc.contributor.committeemember DiVincenzo, John en_US
dc.contributor.department Chemistry en_US
dc.date.accessioned 2014-06-02T18:50:38Z
dc.date.available 2014-06-02T18:50:38Z
dc.date.issued 2013-07-12 en_US
dc.description.abstract A method for detecting the pharmaceutical drug, pentobarbital, in the complex matrix soil has been developed and will be detailed in this thesis. Of growing concern is the release of pharmaceuticals in the environment. Pentobarbital is an organic compound in the barbiturate family that is used often in the euthanasia of animals. Once these animals are buried, pentobarbital may leach into the surrounding soil and become a source of contamination. Satisfactory recoveries of the pentobarbital from soil indicate LC/MS coupled with solid phase extraction is an effective method for analysis and detection. Pre-concentration via solid phase extraction allowed 0.001 mg of pentobarbital per 5 grams of soil (200 ppb) to be detectable at limits of quantification using liquid chromatography/mass spectrometry. This method is suitable for larger quantities of soil and applicable for a wide range of soil types. en_US
dc.description.abstract This method has further applications in determining the decay and dispersion of pentobarbital in soil. Other applications include studying soil bacterium that possess an enzyme reported to be capable of breaking down pentobarbital into its metabolites. en_US
dc.description.degree M.S. en_US
dc.identifier.uri http://jewlscholar.mtsu.edu/handle/mtsu/3573
dc.publisher Middle Tennessee State University en_US
dc.subject Barbiturate en_US
dc.subject Emerging Environmental Contami en_US
dc.subject LC/MS en_US
dc.subject Pentobarbital en_US
dc.subject Soil Bacterium en_US
dc.subject Solid Phase Extraction en_US
dc.subject.umi Chemistry en_US
dc.subject.umi Biochemistry en_US
dc.thesis.degreegrantor Middle Tennessee State University en_US
dc.thesis.degreelevel Masters en_US
dc.title EXTRACTION AND DETECTION OF PENTOBARBITAL IN SOIL BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY / MASS SPECTROMETRY en_US
dc.type Thesis en_US
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