Establishing Tissue Culture of King of Bitters (Andrographis paniculata) and Comparison of Callus Versus Plant Neoandrographolide Production

dc.contributor.authorMihalko, Cassandra
dc.date.accessioned2017-09-07T18:05:16Z
dc.date.available2017-09-07T18:05:16Z
dc.date.issued2017-09
dc.description.abstractNeoandrographolide is a secondary compound endemic to Andrographis paniculata (King of Bitters) and gives the plant its anti-inflammatory property. Because of the widespread use of neoandrographolide and the low seed germination rate of Andrographis paniculata, creating a callus tissue culture, from which to extract neoandrographolide, has the potential to be beneficial to pharmaceutical companies. Callus was produced from King of Bitters plant tissue using the Mineo protocol for tissue extraction and the Murashige protocol for the callus media and incubation. HPLC-CAD analysis was done on a liquid extract of callus (1 g dried callus to 50 mL methanol) and leaf tissue (1g dried leaves to 50 mL methanol) using the Li protocol. No detectable neoandrographolide content was found in the callus, but neoandrographolide was found in the plant leaf tissues. To facilitate neoandrographolide production in callus, elicitors, genetic manipulation, metabolic engineering, or the use of symbionts may be viable options.en_US
dc.identifier.urihttp://jewlscholar.mtsu.edu/xmlui/handle/mtsu/5351
dc.publisherUniversity Honors College, Middle Tennessee State Universityen_US
dc.subjectKing of Bittersen_US
dc.subjectAndrographis paniculataen_US
dc.subjectAndrographolideen_US
dc.subjectneoandrographolideen_US
dc.subjecttissue callusen_US
dc.subjectHPLC-CADen_US
dc.subjectherbal medicineen_US
dc.titleEstablishing Tissue Culture of King of Bitters (Andrographis paniculata) and Comparison of Callus Versus Plant Neoandrographolide Productionen_US
dc.typeThesisen_US

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