Purification and Monosaccharide Composition of Polysaccharides Isolated from Cannabis sativa Cherry Hemp Root
Purification and Monosaccharide Composition of Polysaccharides Isolated from Cannabis sativa Cherry Hemp Root
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Date
2019-04-24
Authors
Dyer, Kami
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Publisher
University Honors College, Middle Tennessee State University
Abstract
Traditional medicine has used plants to reduce inflammation, tumors, and in some cases
the effects of radiation. While these plants tended to be effective, the reason behind their
medicinal properties was unknown. There has now been extensive research on
polysaccharides in many plants regarding their immunomodulatory effects. Hemp has
been shown to have many bioactive properties including anti-anxiety, anti-nausea, antiarthritic,
anti-psychotic, and anti-inflammatory properties. While there has been much
research done on the cannabinoids of hemp, there has been no previous research on the
polysaccharides. This project focuses on extracting the polysaccharides of hemp roots
and determining their monosaccharide composition. The cherry hemp root
polysaccharides were extracted using ethanol and water. The crude extract was then
purified by anion-exchange chromatography and size exclusion chromatography. The
pure polysaccharides were hydrolyzed, and the resulting monosaccharides were
derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP) for HPLC analysis and
Trimethylsilyl (TMS) for GC-MS analysis. This data will be used to help predict
bioactivity, as certain polysaccharide structures have been shown to be
immunostimulatory.
Description
Keywords
monosaccharide,
polysaccharide,
cherry hemp,
chromatography