Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient

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Pellizzaro, Aline
Welker, Gabriel
Scott, David
Solomon, Rance
Cooper, James
Farone, Anthony
Farone, Mary
Mushi, Robert S.
Aguinaga, Maria del Pilar
Erenso, Daniel
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Using a laser trap, we have studied the properties of erythrocytes from a sickle cell anemia patient (SCA) after receiving an intravenous blood transfusion, and a normal adult individual carrying normal adult hemoglobin. The hemoglobin type and quantitation assessment was carried out by high performance liquid chromatography (HPLC). We conducted an analysis of the size distributions of the cells. By targeting those erythrocytes in the overlapping regions of size distributions, we have investigated their properties when the cells are trapped and released. The efficacy of the transfusion treatment is also studied by comparing the relative changes in deformation and the relaxation-time of the cells in the two samples.
Experimental physics, Biology and medicine, Optical confinement and manipulation, Optical tweezers or optical manipulation
Biomedical Optics Express. 2012 Aug 16;3(9):2190