Characteristics of the Structure and Selected Biological Activities of Polysaccharides Isolated from Fedora 17 Hemp (Cannabis sativa)

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University Honors College, Middle Tennessee State University

Abstract

This study focuses on characterizing the structural properties and biological activities of polysaccharides isolated from the Fedora 17 hemp (Cannabis sativa L.). Hemp polysaccharides are an underexplored resource with significant potential in industries such as biotechnology, agriculture, and medicine due to their antioxidant, anti-inflammatory, and immunomodulatory properties. Techniques, including ion-exchange chromatography (IEX), size-exclusion chromatography (SEC), and Fourier-transform infrared spectroscopy (FTIR), were employed to elucidate the molecular weight distribution and structural complexity of the polysaccharides. Additionally, antioxidant activity was assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DDPH) assay. The findings contribute to a broader understanding of hemp polysaccharides' potential applications in the development of sustainable and bioactive products.

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